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Lactobacillus crispatus CTV-05 cells grown under ambient atmosphere were weak and unable to inhibit either Candida strain with or without CNNaS with price match guarantee. In contrast, Lactobacillus crispatus CTV-05 cells grown anaerobically for 24, 48 or 72 hours effectively inhibited growth of both Candida strains. The addition of CNNaS to the medium did not increase the efficiency of inhibition.

The plates were overlayed with YM agar with 10 mM CNNaS sodium thiocyanate, previously reported to be an inhibitor of yeast growth or without CNNaS. The agar overlays were inoculated with one of two strains of Candida albicans ATCC 60193 cervical isolate or Candida albicans ATCC 14053 human blood isolate Zones of inhibition were measured. Control strains of Lactobacillus jensenii CTV-01, Lactobacillus crispatus CTV-02, Lactobacillus crispatus CTV-03, and Lactobacillus crispatus CTV-04 were grown in the same way.

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